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Transformation (genetics): Quiz

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Question 1: In 1944 this "transforming factor" was identified as being genetic by Oswald Avery, Colin MacLeod, and ________.
Roger Y. TsienMaclyn McCartyOliver SmithiesBarbara McClintock

Question 2: For such genomes this method is a form of ________ and not a real transformation, since the inserted genes never reach the nucleus of the cell and do not integrate into the host genome.
Optical transfectionTransfectionHorizontal gene transferTransposon

Question 3: ________ mediated transformation is the easiest and most simple plant transformation.
GenomeBacteriaAgrobacteriumAgrobacterium tumefaciens

Question 4: Chilling cells in the presence of divalent ________ such as Ca2+ (in CaCl2) prepares the cell membrane to become permeable to plasmid DNA.
Half cellElectrolyteLithium-ion batteryIon

Question 5: Introduction of DNA into animal cells is usually called ________, and is discussed in the corresponding article.
TransposonOptical transfectionTransfectionHorizontal gene transfer

Question 6: In ________, transformation is the genetic alteration of a cell resulting from the uptake, genomic incorporation, and expression of environmental genetic material (DNA).
Post-transcriptional regulationMolecular biologyEpigeneticsTranscriptional regulation

Question 7: ________: make transient holes in cell membranes using electric shock; this allows DNA to enter as described above for Bacteria.
Cell (biology)ElectroporationCell wallLipid bilayer

Question 8: ________ is another way to make holes in bacterial (and other) cells, by briefly shocking them with an electric field of 10-20kV/cm.
Lipid bilayerDNACell (biology)Electroporation

Question 9: Transformation was first demonstrated in 1928 by Frederick Griffith, an English ________ searching for a vaccine against bacterial pneumonia.
MicrobiologyNatural sciencePseudoscienceSocial sciences

Question 10: Transformation using ________ was developed in the late 1980's, increasing the efficiency and number of bacterial strains that could be transformed.
Cell (biology)Lipid bilayerElectroporationDNA







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