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Restriction enzyme: Quiz


Question 1: While recognition sequences vary between 4 and 8 nucleotides, many of them are ________, which correspond to nitrogenous base sequences that read the same backwards and forwards.

Question 2: Several enzyme cofactors, including ________ (AdoMet), hydrolyzed adenosine triphosphate (ATP) and magnesium (Mg2+) ions, are required for their activity.
S-Adenosyl methionineTryptophanAcetyl-CoAPhenylalanine

Question 3: A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded ________ at specific recognition nucleotide sequences known as restriction sites.

Question 4: In a similar manner, restriction enzymes are used to digest ________ DNA for gene analysis by Southern blot.

Question 5: [9] Their discovery led to the development of recombinant DNA technology that allowed, for example, the large scale production of human insulin for diabetics using ________ bacteria.
Helicobacter pyloriPseudomonas aeruginosaPathogenic bacteriaEscherichia coli

Question 6: They are used to assist insertion of genes into plasmid vectors during ________ and protein expression experiments.
Genetic engineeringCloning vectorDNA sequencingMolecular cloning

Question 7: Each enzyme is named after the bacterium from which it was isolated using a naming system based on bacterial ________, species and strain.
Class (biology)Biological classificationGenusLife

Question 8: [4][5] Inside a bacterial host, the restriction enzymes selectively cut up foreign DNA in a process called restriction; host DNA is ________ by a modification enzyme (a methylase) to protect it from the restriction enzyme’s activity.
MethylationAmino acidPosttranslational modificationAcetylation

Question 9: The sample is first digested with the restriction enzyme to generate DNA fragments, and then the different sized fragments separated by ________.
SDS-PAGEProteinGel electrophoresisWestern blot

Question 10: Restriction enzymes can also be used to distinguish gene alleles by specifically recognizing single base changes in DNA known as ________ (SNPs).
Introduction to geneticsRNAGeneticsSingle-nucleotide polymorphism


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