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Molecular cloning: Quiz


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Question 1: Following ligation, a portion of the ligation reaction, including vector with insert in the desired orientation is ________ into cells.
TransposonHorizontal gene transferTransfectionOptical transfection

Question 2: Insert preparation is frequently achieved by means of polymerase chain reaction, but it may also be accomplished by restriction enzyme digestion, DNA sonication and fractionation by ________.
SDS-PAGEMolecular biologyAgarose gel electrophoresisEthidium bromide

Question 3: Modern cloning vectors include selectable markers (most frequently ________ resistance markers) that allow only cells in which the vector, but not necessarily the insert, has been transfected to grow.
Antibiotic misuseATC code J01Nucleic acid inhibitorAntibiotic

Question 4: In the classical restriction and ligation cloning protocols, cloning of any DNA fragment essentially involves four steps: DNA fragmentation with ________, ligation of DNA fragments to a vector, transfection, and screening/selection.
PDE2Restriction enzymeR.EcoRIIPhosphatase

Question 5: Following ligation, the ligation product (________) is transformed into bacteria for propagation.
PlasmidBacterial conjugationArchaeaDNA

Question 6: This may be accomplished by means of PCR, restriction fragment analysis and/or ________.
DNA sequencingHuman Genome ProjectFull genome sequencingGenome project

Question 7: Cloning is frequently employed to amplify DNA fragments containing ________, but it can be used to amplify any DNA sequence such as promoters, non-coding sequences, chemically synthesised oligonucleotides and randomly fragmented DNA.


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