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Gel electrophoresis: Quiz

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Question 1: 1959 - introduction of ________ gels; disc electrophoresis (Ornstein and Davis); accurate control of parameters such as pore size and stability; and (Raymond and Weintraub)
AcrylamideEthanolCarbon monoxideAmmonia

Question 2:
Which of the following are related to Gel electrophoresis?

Question 3: "Electrophoresis" refers to the ________ (EMF) that is used to move the molecules through the gel matrix.
Electromotive forceElectromagnetismElectric currentMaxwell's equations

Question 4: 1955 - introduction of ________ gels, mediocre separation
StarchSucroseGlucoseCellulose

Question 5: In the case of nucleic acids, the direction of migration, from negative to positive electrodes, is due to the naturally-occurring negative charge carried by their sugar-________ backbone.
PhosphatePhosphate homeostasisPhosphorusAdenosine triphosphate

Question 6: If the analyte molecules ________ under ultraviolet light, a photograph can be taken of the gel under ultraviolet lighting conditions.
FluorescenceFluorescent lampX-rayLight-emitting diode

Question 7: ________ is composed of long unbranched chains of uncharged carbohydrate without cross links resulting in a gel with large pores allowing for the separation of macromolecules and macromolecular complexes.
FungusGrowth mediumSeaweedAgar

Question 8: If the molecules to be separated contain ________ added for visibility, an autoradiogram can be recorded of the gel.
Gamma rayRadioactive decayNuclear fissionNuclear fusion

Question 9: Gel electrophoresis is a technique used for the separation of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein ________ using an electric field applied to a gel matrix.
MoleculeNeutronAtomElectron

Question 10: ________, in contrast to polyacrylamide, is a neurotoxin and must be handled using appropriate safety precautions to avoid poisoning.
AmmoniaEthanolCarbon monoxideAcrylamide







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