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GC-content: Quiz


Question 1: The absorbance of DNA at a wavelength of 260 nm increases fairly sharply when the double-stranded ________ separates into two single strands when sufficiently heated.

Question 2: G (guanine) and C (cytosine) undergo a specific ________ whereas A (adenine) bonds specifically with T (thymine).
Dipolar bondChemical polarityHydrogen bondChemical bond

Question 3: The GC pair is bound by three ________, while AT pairs are bound by two hydrogen bonds.
Chemical polarityHydrogen bondChemical bondDipolar bond

Question 4: [20] Because of the nature of the ________, it is virtually impossible for an organism to have a genome with a GC-content approaching either 0% or 100%.
Genetic codeDNAProteinAmino acid

Question 5: [7] The most commonly used protocol for determining GC ratios uses ________ for large number of samples.
InflammationFlow cytometryImmunohistochemistryHistopathology

Question 6: GC-content (or guanine-cytosine content), in molecular biology, is the percentage of nitrogenous bases on a ________ molecule which are either guanine or cytosine (from a possibility of four different ones, also including adenine and thymine).

Question 7: A species with an extremely low GC-content is ________ (GC% = ~20%),[21] and it is usually common to refer to such examples as being AT-rich instead of GC-poor.
Plasmodium ovalePlasmodium falciparumPlasmodium vivaxMalaria

Question 8: In ________ experiments, the GC-content of primers are used to predict their annealing temperature to the template DNA.
Taq polymeraseReverse transcription polymerase chain reactionReal-time polymerase chain reactionPolymerase chain reaction

Question 9: The GC-content percentages as well as GC-ratio can be measured by several means but one of the simplest methods is to measure what is called the melting temperature of the ________ double helix using spectrophotometry.

Question 10: [1] This may refer to a specific fragment of DNA or ________, or that of the whole genome.
Non-coding RNARNAMessenger RNASmall interfering RNA


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