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Chromatography: Quiz

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Question 1: Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as ________ (HPLC).
Gas-liquid chromatographyHigh performance liquid chromatographyLiquid chromatography-mass spectrometrySize exclusion chromatography

Question 2: In conventional methods the stationary phase is an ion exchange resin that carries charged ________ which interact with oppositely charged groups of the compound to be retained.
EthyleneAlkaneAlcoholFunctional group

Question 3: However, instead of using a stationary phase of paper, it involves a stationary phase of a thin layer of adsorbent like silica gel, alumina, or ________ on a flat, inert substrate.
CelluloseSucroseStarchGlucose

Question 4: Chromatography, literally "color writing", was used—and named— in the first decade of the 20th century, primarily for the separation of plant pigments such as ________.
Plant physiologyChloroplastPhotosynthesisChlorophyll

Question 5: In the case of ________ the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or polar solvents in reverse phase chromotagraphy and the sample being separated.
High performance liquid chromatographyGas-liquid chromatographyChromatographySize exclusion chromatography

Question 6: The paper is placed in a jar containing a shallow layer of ________ and sealed.
SolventSolutionSolubilitySolvation

Question 7: Subtle differences in compounds ________ results in differential retention on the stationary phase and thus changing the separation.
Partition coefficientSolubilitySolubility equilibriumSolvent

Question 8: Examples include the ________ layer in thin layer chromatography
Silicon dioxideAmethystOpalQuartz

Question 9: It is often used in biochemistry in the purification of ________ bound to tags.
MetabolismMyosinProteinActin

Question 10: Ion exchange chromatography uses a charged stationary phase to separate charged compounds including ________, peptides, and proteins.
Amino acid synthesisMetabolismAmino acidL-DOPA







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